A dual-response BODIPY-based fluorescent probe for the discrimination of glutathione from cystein and homocystein† †Electronic supplementary information (ESI) available: Synthesis, spectroscopic properties, NMR and mass spectra. See DOI: 10.1039/c5sc00216h Click here for additional data file.
نویسندگان
چکیده
1. Time dependent spectral changes of BODIPY-S in the presence of thiols. ......S2 2. MS of BODIPY-S with GSH, Cys and Hcy..............................................S3 3. Photophysical spectra of BODIPY 4 and BODIPY-S +Cys..........................S4 4. Time dependent Spectral changes of S-S-BODIPY in the presence of thiols. ...S5 5. NMR and MS of the product of S-S-BODIPY with thiols.............................S6 6. Reaction mechanism .................................................................... ... ..S7 7. Pseudo first-order kinetic plot..............................................................S8 8. HRMS of S-S-BODIPY-S with thiols ...............................................S9-S10 9. Time-dependent spectral changes of S-S-BODIPY-S with Cys/Hcy...............S11 10. The easy-to-monitor fluorescence color change......................................S12 11. Fluorescent confocal image of cells. ....................................................S12 12. Experimental section..................................................................S13-S15 13. NMR and HRMS spectra............................................................S16-S21
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Department of Biology and Chemistry, Cit Avenue, Kowloon, Hong Kong, China. E-m cityu.edu.hk Key Laboratory of Biochip Technology, Biote Institute of City University of Hong Kong, Sh Key Laboratory of Flexible Electronics & Inst University, 30 South Puzhu Road, Nanjing, Department of Chemistry, National Univers † Electronic supplementary information ( properties, NMR, mass spectra and conf and ...
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